OMICs-based investigations of microorganisms are becoming more and more widespread in the upcoming era of systems and synthetic biology. Here, proteomics plays a key role and two-dimensional difference gel electrophoresis (2D DIGE) remains the “gold-standard” for globally determining protein abundance changes on a quantitative and statistically confident level—in particular also for laboratories not having full-cycle proteomic facilities at their disposal. In this contribution we summarize our methodological procedures and experiences with 2D DIGE accumulated over the past 15 years.
CITATION STYLE
Trautwein, K., & Rabus, R. (2018). Applications of difference gel electrophoresis (DIGE) in the study of microorganisms. In Methods in Molecular Biology (Vol. 1841, pp. 95–112). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8695-8_8
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