Peripheral binding of proteins to lipid bilayers is critical not only in intracellular signaling but also in metalloproteinase shedding of signaling proteins from cell surfaces. Assessment of how proteins recognize fluid bilayers peripherally using crystallography or structure-based predictions has been important but incomplete. Assay of dynamic protein–bilayer interactions in solution has become feasible and reliable using paramagnetic NMR and site-directed fluor labeling. Details of preparations and assay protocols for these spectroscopic measurements of bilayer proximity or contact, respectively, are described.
CITATION STYLE
Marcink, T. C., Koppisetti, R. K., Fulcher, Y. G., & Van Doren, S. R. (2017). Mapping lipid bilayer recognition sites of metalloproteinases and other prospective peripheral membrane proteins. In Methods in Molecular Biology (Vol. 1579, pp. 61–86). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6863-3_5
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