Preparation of nanocones for immobilizing DNA probe by a low-temperature plasma plume

Abstract

Using allylamine monomer, a matrix of nanocones was fabricated by applying a low-temperature plasma plume without any catalysts and template. This nanocone acted as an adhesion layer immobilizing DNA probe for DNA hybridization assay. A simple conceptual model to describe the growth of the nanocones was also developed. The highest density of amino-labeled DNA probe was about 1.6pM/cm 2 confirmed by the dyed oligonucleotide, and each nanocone contained nearly 3 times; 102 amine groups. This strategy provides a robust procedure to immobilize DNA, which is a very useful substrate for fabricating nanobiosensors. © 2006 American Institute of Physics.