Solution to the high Dissolved CO2 Problem in High-Density Perfusion Culture of Mammalian Cells

Abstract

High dissolved CO2 concentration (DCO2) is a major problem causing growth inhibition, metabolic changes, and poor productivity in mammalian cell culture. DCO2 often accumulates up to 200∓250 mmHg in perfusion bioreactors, while the optimal physiological range is typically 40--50 mmHg. Although the problem with CO2 inhibition is well recognized, this critical parameter is generally not systematically controlled in bioreactor systems.