Antifeedant activity of luteolin and genistein against the pea aphid, Acyrthosiphon pisum

Abstract

Electrical penetration graphs (DC EPG) were used to monitor the feeding behavior of the pea aphid, Acyrthosiphon pisum Harris (Hemiptera: Aphididae) exposed to the flavonoids luteolin and genistein in artificial diets. The EPG patterns generated by aphids feeding on plants were used to interpret the patterns generated on the artificial diets. Addition of flavonoids to the diets generally prolonged the period of stylet probing (as indicated by EPG pattern d-C), reduced salivation (as indicated by pattern d-E1) and passive ingestion (as indicated by pattern d-E2), and also delayed the onset of salivation and passive ingestion. At higher concentrations (≥100 μg cm-3 for luteolin, ≥1,000 μg cm-3 for genistein), the flavonoids completely stopped salivation and passive ingestion. In most events associated with active ingestion (EPG pattern d-G), however, differences in feeding behavior did not statistically differ between the control diet and those with flavonoids; luteolin, and genistein only at 10 μg cm-3 prolonged the time until the first d-G pattern was observed. The current findings demonstrate detrimental effects of the isoflavone genistein and the flavone luteolin on the feeding behavior of the pea aphid, A. pisum. This can be employed to create plants which are resistant to aphids and other herbivores. © 2012 The Author(s).