The 5' fluorogenic (TaqMan) assay has been successfully used in screening for single-nucleotide polymorphisms; the very few steps required and the ability to automate each step allow for high-throughput screening. Insertion/deletion polymorphisms are an important class of markers that can be studied for different applications, such as diagnostics, genome variation, and species identification. Polymerase chain reaction (PCR) and post-PCR analysis are required to score the insertion or the deletion allele. In this chapter, we describe an expansion of the TaqMan technology for a rapid, high-throughput, screening for insertion/deletion polymorphisms in which the exact endpoints are known. The method requires minimal post-PCR analysis and can be applied to polymorphisms of any size.
CITATION STYLE
Robledo, R., Beggs, W. R., & Bender, P. K. (2005). TaqMan genotyping of insertion/deletion polymorphisms. Methods in Molecular Biology (Clifton, N.J.), 311, 165–176. https://doi.org/10.1385/1-59259-957-5:165
Mendeley helps you to discover research relevant for your work.