Optimización del método SCAR (Sequence Characterized Amplified Region) que favorece el aislamiento de loci polimórficos para estudios filogenéticos en taxa cercanamente relacionados

Abstract

DNA sequence data have made a tremendous contribution to plant phylogenetics. Nonetheless, a notable shortcoming of several loci has been the poor phylogenetic resolution of relationships among closely related species. In this study a modification to die SCAR method is proposed that consists of: a) digestion of polymorphic RAPD fragments instead of cloning them, b) sequencing of the digested fragments, and c) alignment of sequences for designing specific primers. This simple approach allowed us to find variable DNA loci suitable for phylogenetic analysis at lower taxonomic ranks.