Crystallization and structure determination of superantigens and immune receptor complexes

Abstract

Structure determination of superantigens and the complexes they form with immune receptors have over the years provided insight in their modes of action. This technique requires growing large and highly ordered crystals of the superantigen or receptor–superantigen complex, followed by exposure to X-ray radiation and data collection. Here, we describe methods for crystallizing superantigens and superantigen–receptor complexes using the vapor diffusion technique, how the crystals may be optimized, and lastly data collection and structure determination.