Intrathymic injection is used in several T cell-associated immunological studies to deliver cells or other substances directly into the thymus. Here, we describe the intrathymic injection procedure involving surgical incision of the mouse with or without a thoracotomy. Though this procedure can result in poor recovery, postsurgical complications, and distress to the animal, it is actually a simple procedure that can be carried out relatively easily and quickly with experience.
CITATION STYLE
Manna, S., & Bhandoola, A. (2015). Intrathymic injection. In T-Cell Development: Methods and Protocols (pp. 203–209). Springer New York. https://doi.org/10.1007/978-1-4939-2809-5_17
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