Cloning and expression of a Bacillus licheniformis α-amylase gene in Pseudomonas aeruginosa

Abstract

The gene for B. licheniformis α-amylase has been cloned in P. aeruginosa. Synthesis of the enzyme occurs in late log phase and goes on during stationary phase. Although P. aeruginosa is a secretory bacterium, α-amylase is not efficiently secreted into the extracellular medium; 85% of the enzyme is retained in the periplasm. © 1985.