Single-cell sequencing of TCR alleles enables determination of T cell specificity. Here we describe a sensitive protocol for targeted amplification of TCR CDR3 regions from single-cell full-length cDNA libraries. By exploiting the specificity of RNase H-dependent PCR (rhPCR), the protocol achieves amplification of TCR alleles and addition of cell barcodes in a single PCR step.
CITATION STYLE
Li, S., & Livak, K. J. (2019). Targeted TCR amplification from single-cell cDNA libraries. In Methods in Molecular Biology (Vol. 1979, pp. 197–224). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9240-9_13
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