Immunoprecipitation-based analysis of protein–protein interactions

Abstract

Several techniques allow the detection of protein–protein interactions. In vivo co-immunoprecipitation (Co-IP) studies are an important complement to other commonly used techniques such as yeast twohybrid or fl uorescence complementation, as they reveal interactions between functional proteins at physiological relevant concentrations. Here, we describe an in vivo Co-IP approach using either GFP affi nity matrix or specifi c antibodies to purify proteins of interests and their interacting partners.