The normal function of neurons depends on the integrity of microtubule-dependent transport of cellular materials and organelles to/from their cell bodies or axon terminus. In this chapter, we describe the design and implementation of a fluorescence imaging method to visualize axonal transport in neurons directly. We combine a pseudo total internal reflection microscopy, quantum dot fluorescence labeling, microfluidic neuronal culture chamber, and single molecule detection methods to achieve a high spatial and temporal resolution in tracking nerve growth factor transport in dorsal root ganglia neurons. © 2011 Springer Science+Business Media, LLC.
CITATION STYLE
Osakada, Y., & Cui, B. (2011). Real-time visualization of axonal transport in neurons. Methods in Molecular Biology, 670, 231–243. https://doi.org/10.1007/978-1-60761-744-0_16
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