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Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA gene-targeted PCR

Applied and Environmental Microbiology (1996) 62(4) 1478-1480
DOI: 10.1128/aem.62.4.1478-1480.1996
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Abstract

A method based on 16S rRNA gene-targeted PCR and oligonucleotide probing was developed for detecting Mycobacterium chlorophenolicum PCP-1 in soil. The primers and probe were specific for PCP-1 in DNA extracts of three soils. The method allowed for PCP-1 detection in soil with a detection limit of 3 x 102 cells per g.

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APA

Briglia, M., Eggen, R. I. L., De Vos, W. M., & Van Elsas, J. D. (1996). Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA gene-targeted PCR. Applied and Environmental Microbiology, 62(4), 1478–1480. https://doi.org/10.1128/aem.62.4.1478-1480.1996

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