The standard approach for the isolation of vaccinia virus recombinants involves homologous recombination between a transfected plasmid and the replicating viral DNA. In a typical infection/transfection experiment, recombinant viruses only account for a tiny proportion (10-4 to 10-3) of the progeny virus; thus, genetic markers are often included in the transfected plasmid to facilitate the selection of recombinant viruses. This chapter describes in detail two different selection procedures: one relies on plaque formation phenotype using the vaccinia virus gene F13L; the other relies on antibiotic resistance using the Escherichia coli xanthine-guanine phosphoribosyl transferase gene.
CITATION STYLE
Lorenzo, M. M., Galindo, I., & Blasco, R. (2004). Construction and isolation of recombinant vaccinia virus using genetic markers. Methods in Molecular Biology (Clifton, N.J.). https://doi.org/10.1385/1-59259-789-0:015
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