Positron emission tomography (PET) using 89Zr is a clinically relevant imaging modality that enables long–term monitoring of adoptively transferred immune cells. This article describes a two-step radiometal labeling procedure utilizing the bifunctional siderophore p-isothiocyanatobenzyl-desferrioxamine (DFO-Bz-NCS) that chelates 89Zr with high affinity and binds covalently to primary amines of cell-surface proteins via its isothiocyanate moiety. Cells labeled with 89Zr-DFO-Bz-NCS remain viable and retain the radiolabel, enabling repetitive PET imaging of adoptively transferred immune cells with high sensitivity and specificity for up to 2 weeks.
CITATION STYLE
Najjar, A. M. (2020). Radiolabeling and imaging of adoptively transferred immune cells by positron emission tomography. In Methods in Molecular Biology (Vol. 2097, pp. 267–272). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0203-4_17
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