STUDIES ON JOINT TOXIC EFFECTS OF A GLYPHOSATE HERBICIDE (FOZÁT 480) AND A HEAVY METAL (CADMIUM) ON CHICKEN EMBRYOS

Abstract

The aim of the study was to determine the individual and combined toxic effects ofthe herbicide Fozát 480 (glyphosate [isopropylamine salt] 480g/l) and cadmiumsulphate (CdSO4) on the development of chicken embryos. On the first day ofincubation, chicken eggs were injected with 0.1 ml of cadmium sulphate solution(0.1%) and/or with 0.1 ml of Fozát 480 (2.0%). The chicken embryos wereexamined on the 19th day by measuring the rate of embryo mortality and bodyweight and by identification of different types of developmental anomalies andmacroscopic malformations. The body weight data were statistically evaluated byone-way ANOVA and Dunnett tests, while the embryonic mortality and thedevelopmental anomalies were analysed by Fisher test. Our teratogenicity studyrevealed, that the combined administration of cadmium sulphate and glyphosate(isopropylamine salt) containing herbicide formulation caused a significantreduction in the body weight of embryos and increased the rate of embryonicmortality. The joint toxic effect of cadmium sulphate and Fozát 480 is an additiveeffect compared to the individual toxicity of the test materials.