Regulatory macrophages (M regs) are a novel type of suppressor macrophage which may be a particularly suitable cell for inducing tolerance of solid organ transplants. In this article, we provide a detailed description of the generation of human M regs from peripheral blood monocytes and methods for the assessment of their phenotype. The uniqueness of the human M reg is best appreciated when the M reg is compared to macrophages in other states of activation; therefore, protocols are provided for generating five comparator macrophage types which have been used as cell type-specificity controls in our work.
CITATION STYLE
Hutchinson, J. A., Riquelme, P., Geissler, E. K., & Fändrich, F. (2011). Human Regulatory Macrophages. In Methods in Molecular Biology (Vol. 677, pp. 181–192). Humana Press Inc. https://doi.org/10.1007/978-1-60761-869-0_13
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