Fmoc solid-phase peptide synthesis (SPPS) is the most common approach used to synthesize natural and unnatural peptides. However, the synthesis of sequences longer than 30–60 amino acids is associated with a drastic reduction in peptide quality. Thus, large and complex peptides are normally synthesized as fragments, which are then conjugated together. Here, we describe the synthesis of a large, branched peptide, a multi-epitope vaccine candidate against Group A Streptococcus, with the help of microwave-assisted Fmoc-SPPS, thiol-maleimide conjugation, and copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC) “click” reaction.
CITATION STYLE
Dai, C., Stephenson, R. J., Skwarczynski, M., & Toth, I. (2020). Application of Fmoc-SPPS, thiol-maleimide conjugation, and copper(i)-catalyzed alkyne-azide cycloaddition “click” reaction in the synthesis of a complex peptide-based vaccine candidate against group a Streptococcus. In Methods in Molecular Biology (Vol. 2103, pp. 13–27). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0227-0_2
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