Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of hyperpolarized 13C-NMR using quantitative dissolution Dynamic Nuclear Polarization (qdDNP) and isotope enrichment add sensitivity to such metabolic studies, enabling mapping and quantification of metabolic pathways and networks. Here we describe a sample preparation method, including cell incubation, extraction, and signal enhancement, for reproducible and quantitative analysis of hyperpolarized 13C-NMR metabolite spectra. We further illustrate how qdDNP can be applied to gain metabolic insights into living cells.
CITATION STYLE
Lerche, M. H., Karlsson, M., Ardenkjær-Larsen, J. H., & Jensen, P. R. (2019). Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization. In Methods in Molecular Biology (Vol. 2037, pp. 385–393). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9690-2_21
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