Bioaccumulation and biotransformation of plasticisers diisononyl phthalate and di(2-ethylhexyl) terephthalate in black soldier fly larvae reared on (micro)plastic-contaminated food waste

Abstract

Food waste is currently used for the production of biogas. However, a reusage of waste is preferred to follow the principles of the circular economy and consider the waste management hierarchy, which can be achieved by rearing black soldier fly (BSF) larvae on such organic waste. Nonetheless, the presence of (micro)plastics and related additive plasticisers might induce chemical safety hazards to the larval applications as feed. Therefore, the bioaccumulation and biotransformation of two plasticisers (diisononyl phthalate (DINP) and di(2-ethylhexyl) terephthalate (DEHT)) in BSF larvae reared on food waste streams contaminated with polyvinyl chloride (PVC) (micro)plastics were investigated. Results showed that BSF larvae appeared to have a moderate intake of DINP during its rearing phase of 10 days (82-273 ng/g), while being able to biotransform it into the primary biotransformation product monoisononyl phthalate (MINP) within 24 h. For DEHT, an uptake of 67-137 ng/g was measured in the BSF larvae; however, no clear biotransformation pattern was observed. In addition, while no secondary oxidative biotransformation products were found in the larvae, these were measured in the frass, leading to the hypothesis that microorganism-mediated biotransformation of plasticisers occurred. In conclusion, based on the results of this study, BSF larvae could potentially be used safely in the frame of circular economy, when reared on organic substrates contaminated with the same PVC microplastic content and sizes used.