DNA-PAINT super-resolution imaging for nucleic acid nanostructures

Abstract

Far-field super-resolution fluorescence microscopy has allowed observation of biomolecular and synthetic nanoscale systems with features on the nanometre scale, with chemical specificity and multiplexing capability. DNA-PAINT (DNA-based point accumulation for imaging in nanoscale topography) is a super- resolution method that exploits programmable transient hybridization between short oligonucleotide strands, and allows multiplexed, single-molecule, single-label visualization with down to ~5-10 nm resolution. DNAPAINT provides a method for structural characterisation of nucleic acid nanostructures with high spatial resolution and single-strand visibility.