Type I RIPs have not been identified in oil palm. EgRIP-1a and EgRIP-1b transcripts were partially isolated from roots and basal stems of oil palm seedlings with high similarity to type I RIPs. Expression levels were altered during plant-pathogen interaction and their proteins were 28-30 kD with an estimated pI value of 10.0. Depurination of yeast 26S rRNA by EgRIP proteins demonstrated specific ribosome-inactivating activity. EgRIP proteins showed inhibition on G. boninense mycelial growth with 44.1% at 5 DAI. It was concluded that the novel EgRIPs are type I and demonstrated to have antifungal effect against G. boninense.
CITATION STYLE
Sargolzaei, M., Ho, C. L., & Wong, M. Y. (2016). Characterization of novel type I ribosome-inactivating proteins isolated from oil palm (Elaeis guineensis) inoculated with Ganoderma boninense, the causal agent of basal stem rot. Physiological and Molecular Plant Pathology, 94, 53–61. https://doi.org/10.1016/j.pmpp.2016.04.002
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