Gene cloning and characterization of a novel cellulose-binding β- glucosidase from Phanerochaete chrysosporium

Abstract

Analysis of a 2.4-kb cDNA of the cellulose-binding extracellular β- glucosidase (CBGL) from Phanerochaete chrysosporium suggested that CBGL is organized into two domains, an N-terminal cellulose-binding domain and a C- terminal catalytic domain. Genomic sequence analysis suggested that cbgl is encoded by 30 exons. Southern analysis of DNA from homokaryotic cultures indicated that CBGL is encoded by two alleles, cbgl-1 and cbgl-2, of a single gene.