CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chronic lymphocytic leukemia. Our approach reliably quantifies mutational co-occurrences, zygosity status, and the occurrence of Cas9 edits at single-cell resolution.
CITATION STYLE
ten Hacken, E., Clement, K., Li, S., Hernández-Sánchez, M., Redd, R., Wang, S., … Wu, C. J. (2020). High throughput single-cell detection of multiplex CRISPR-edited gene modifications. Genome Biology, 21(1). https://doi.org/10.1186/s13059-020-02174-1
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