Transcriptome-wide mapping RNA modification is crucial to understand the distribution and function of RNA modifications. Here, we describe a protocol to transcriptome-wide mapping 5-methylcytosine (m5C) in plant, by a RNA immunoprecipitation followed by deep sequencing (m5C-RIP-seq) approach. The procedure includes RNA extraction, fragmentation, RNA immunoprecipitation, and library construction.
CITATION STYLE
Gu, X., & Liang, Z. (2019). Transcriptome-Wide Mapping 5-Methylcytosine by m5C RNA Immunoprecipitation Followed by Deep Sequencing in Plant. In Methods in Molecular Biology (Vol. 1933, pp. 389–394). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9045-0_24
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