Evaluation of dot-elisa for serodiagnosis of fasciolosis in naturally infected sheep

Abstract

In order to evaluate dot-ELISA technique for serodiagnosis of ovine fasciolosis, 40 sera samples from infected and 10 as control from non infected sheep were collected from a local abattoir. Somatic antigens, and excretory / secretory (E / S) products ofFasciola were prepared. The nitrocellulose membrane disks (5 mm diameter) were coated with antigens and blocked with 3% skimmed milk. Then they were washed and incubated with sera for 60 min. The anti sheep immunoglobulin conjugate was used at 1:1000 dilutions for 60 min and incubated using DAB substrate. Disks were analysed at room temperature for the presence of any visible dot. Specificity and sensitivity of somatic and EIS antigens were 94.6%, 58.4% and 100%, 75%, respectively. No cross reaction was observed with Dicrocoelium dendriticum, Hydatid cyst and Cysticercus tenuicollis. It is concluded that dot-ELISA system by using E/S antigen is a useful mean to detect fasciolosis in sheep. © GSP, India.