High-dimensional modeling for cytometry: Building rock solid models using GemStone™ and verity cen-se’™ high-definition t-SNE mapping

Abstract

This chapter outlines how to approach the complex tasks associated with designing models for high-dimensional cytometry data. Unlike gating approaches, modeling lends itself to automation and accounts for measurement overlap among cellular populations. Designing these models is now easier because of a new technique called high-definition t-SNE mapping. Nontrivial examples are provided that serve as a guide to create models that are consistent with data.