In vivo bioluminescent imaging of Yersinia ruckeri pathogenesis in fish

Abstract

Bioluminescent reporters and advanced luciferase technologies are useful to study host–pathogen interactions. This chapter describes the use of the luxCDABE operon from Photorhabdus luminescens as a tool to analyze the progression of the fish pathogen Yersinia ruckeri during the infection of rainbow trout, as well as the quantification of promoter activity of specific bacterial genes during host colonization.