Quartz crystal microbalance with dissipation monitoring (QCM-D) is a useful technique for observing the adsorption of molecules onto a protein-functionalized surface in real time. This technique is based on relating changes in the frequency of a piezoelectric sensor chip, onto which molecules are adsorbing, to changes in mass using the Sauerbrey equation. Here, we outline the cleaning, preparation, and analysis involved in a typical QCM-D experiment, from which one can obtain mass adsorption and kinetic binding information.
CITATION STYLE
Heller, G. T., Mercer-Smith, A. R., & Johal, M. S. (2015). Quartz microbalance technology for probing biomolecular interactions. Methods in Molecular Biology, 1278, 153–164. https://doi.org/10.1007/978-1-4939-2425-7_9
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