Development and characterization of common wheat-Thinopyrum intermedium translocation lines with resistance to barley yellow dwarf virus

Abstract

We developed some wheat-Th. intermedium translocation lines, Yw642, Yw443 and Yw243, etc., showing good BYDV resistance from L1 by induced homoeologous pairing using CS ph mutant. Characterization of these wheat lines was carried out by GISH and RFLP analysis. The results of GISH showed that the lines, Yw642, Yw443 and Yw243, etc., are homozygous wheat-Th. intermedium translocation lines, in which the chromosome segments of Th. intermedium were transferred to the distal end of a pair of wheat chromosomes. RFLP analysis indicated that the translocation chromosome of the wheat lines is T7DS·7DL-7XL. The breakpoint of the translocation is located on the distal end of 7DL, between Xpsr965 and Xpsr680 about 90-99 cm from the centromere. The BYDV gene is located on the distal end of 7XL around Xpsr680, Xpsr687 and Xwg380. The RFLP markers of psr680, psr687 and wg380 were cosegregated with the BYDV resistance respectively and could be used for molecular assisted selection (MAS) in wheat breeding program for BYDV resistance.