Functional, quantitative, and super-resolution imaging and spectroscopic approaches for studying exocytosis

0Citations
Citations of this article
4Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Regulated exocytosis is the process of secretion in specialized cells. Decades of intensive research have defined the molecules that drive the process of membrane fusion, as well as a plethora of accessory factors that shape and modulate the exocytotic function. As regulated exocytosis is generally a rapid event with a millisecond timescale, involving organelles on the nanometer scale, the field has long employed high spatial and temporal resolution techniques, including electrophysiology and, importantly, imaging approaches. Huge gains in the spatial and temporal resolution in biological microscopy have been delivered by recent hardware, engineering, and software breakthroughs; it is now possible to visualize and quantity the distributions, movements, and interactions of large cohorts of single protein molecules inside living cells. These technically demanding approaches have the potential to test long-standing hypotheses in the fields of membrane trafficking and exocytosis, building upon an extraordinary foundation of detailed biochemical and electrophysiological understanding. © Springer Science+Business Media New York 2014.

Cite

CITATION STYLE

APA

Duncan, R. R., & Rickman, C. (2014). Functional, quantitative, and super-resolution imaging and spectroscopic approaches for studying exocytosis. Neuromethods. Humana Press Inc. https://doi.org/10.1007/978-1-62703-676-4_5

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free