Genetic polymorphism among red jungle fowl and domesticated chicken detected by dna markers

Abstract

Genetic polymorphism was detected between Red Jungle Fowl (RJF), White Leghorn (WL), Rhodes Island Red (RIR), White Plymouth Rock (WR), Red Cornish (RC), Aseel (AS) and Kadaknath (KN) using 3 DNA marker systems i.e. Randomly Amplified Polymorphic DNA Polymorphism (RAPD, 20 primers), Microsatellite / Minisatellite Associated Sequence Amplification (MASA, 3 primers) and Microsatellite markers (8 primers). Out of 119 bands amplified from the 18 polymorphic RAPD primers, 48% were polymorphic. Using MASA primers a total of 17 loci were amplified and about 39% of them were polymorphic. Out of 8 micro satellite markers, five markers were polymorphic. Number of alleles amplified with different microsatellite markers ranged from 3 to 6 with an average of 5.0 alleles per marker. Using three marker assay, comparatively lower genetic similarity was estimated between RJF and other domestic breeds, among whom, native breeds, specially Aseel was comparatively closer to RJF. © GSP, India.