A novel convenient method for high bacteriophage titer assay.

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Abstract

The recent various applications of phages (bacteriophages) including phage therapy have brought about a revival of phage investigation. The phage titer assay is indispensable for phage experiments. However, the conventional standard method is a plaque counting method which requires a little skill with tedious repeating operation. Furthermore, it is not directly applicable to high phage titers. In this paper, we describe a novel convenient "cross streak and paper disk assay method" for high titer concentration without plaque counting.

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Yoshida, K., Nasu, Y., Shitami, N., Toyoda, H., Takemura, H., & Oomori, K. (2009). A novel convenient method for high bacteriophage titer assay. Nucleic Acids Symposium Series (2004), (53), 315–316. https://doi.org/10.1093/nass/nrp158

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