Genetic manipulation of bacteria often requires the joining together of more than one DNA segment to form a hybrid DNA molecule. This can be accomplished by PCR followed by restriction endonuclease digestions and ligations. However, this approach can often become laborious and expensive. Here is described a well-established method for using primer design and PCR to obtain hybrid products for use in cloning vectors, mutagenesis protocols, and other applications.
CITATION STYLE
Thornton, J. A. (2016). Splicing by overlap extension pcr to obtain hybrid dna products. In Methods in Molecular Biology (Vol. 1373, pp. 43–49). Humana Press Inc. https://doi.org/10.1007/7651_2014_182
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