The visible photodissociation mechanisms of QSY7-tagged peptides of increasing size have been investigated by coupling a mass spectrometer and an optical parametric oscillator laser beam. The experiments herein consist of energy resolved collision- and laser-induced dissociation measurements on the chromophore-tagged peptides. The results show that fragmentation occurs by similar channels in both activation methods, but that the branching ratios are vastly different. Observation of a size-dependent minimum laser pulse energy required to induce fragmentation, and collisional cooling rates in time resolved experiments show that laser-induced dissociation occurs through the absorption of multiple photons by the chromophore and the subsequent heating through vibrational energy redistribution. The differences in branching ratio between collision- and laser-induced dissociation can then be understood by the highly anisotropic energy distribution following absorption of a photon. [Figure not available: see fulltext.].
CITATION STYLE
Bouakil, M., Kulesza, A., Daly, S., MacAleese, L., Antoine, R., & Dugourd, P. (2017). Visible Multiphoton Dissociation of Chromophore-Tagged Peptides. Journal of the American Society for Mass Spectrometry, 28(10), 2181–2188. https://doi.org/10.1007/s13361-017-1733-9
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