Bioreactor Process Strategies for rNS0 Cells

Abstract

rNS0 cell line has been grown in protein-free, chemically-defined medium using stirred-tank bioreactors in batch and fed-batch mode. Various operating parameters, including pH and temperature, were investigated to assess their effect on growth and IgG production in a chemically defined, protein-free culture system. Spent medium analysis was used to determine the effect of bioreactor operating parameters on nutrient metabolism and IgG production. To increase the total yield, optimum bioreactor operating conditions found in batch culture were then applied to a fed-batch process. The effect of bioreactor conditions on nutrient utilization rates and feeding strategy will be discussed.