LC-mass spectrometry for metabolomics

Abstract

The field of metabolomics is greatly being refined by the addition of new technologies. LC-MS has allowed researchers to explore additional metabolites which were not originally captured through GC-MS. Through the customizability of the LC columns and mass spectrometer, it is now easier to tailor the instrument to your research needs. Herein, we describe a protocol for sample preparation and data acquisition for a global metabolomic analysis of tissues or feces.