We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for yeast and another for filamentous fungi. Left and right borders were added for Agrobacterium tumefaciens recognition, and a minimal broad-host range RK2 replication origin. Transformation was validated in the pathogenic fungus Paracoccidioides lutzii. Hence, we developed an efficient and reliable molecular tool for fungal transformation: minimalist, synthetic, modular, and available in four different versions, and these can still be readily modified using a few primers and few cloning steps.
CITATION STYLE
Nora, L. C., Gonçales, R. A., Martins-Santana, L., Ferreira, B. H., Rodrigues, F., & Silva-Rocha, R. (2019). Synthetic and minimalist vectors for agrobacterium tumefaciens-mediated transformation of fungi. Genetics and Molecular Biology, 42(2), 395–398. https://doi.org/10.1590/1678-4685-gmb-2018-0221
Mendeley helps you to discover research relevant for your work.