Pathologic prion protein infects cells by lipid-raft dependent macropinocytosis

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Abstract

Transmissible spongiform encephalopathies, including variant-Creutzfeldt-Jakob, disease (vCJD) in humans and bovine spongifom encephalopathies in cattle, are fatal neurodegenerative disorders characterized by protein misfolding of the host cellular prion protein (PrPC) to the infectious scrapie form (PrPSc). However, the mechanism that exogenous PrPSc infects cells and where pathologic conversion of PrPC to the PrPSc form occurs remains uncertain. Here we report that similar. to the mechanism of HIV-1 TAT-mediated peptide transduction, processed mature, full length PrP contains a conserved N-terminal cationic domain that stimulates cellular uptake by lipid raft-dependent, macropinocytosis. Inhibition of macropinocytosis by three independent means prevented cellular uptake of recombinant PrP; however, it did not affect recombinant PrP cell surface association. In addition, fusion of the cationic N-terminal PrP domain to a Cre recombinase reporter protein was sufficient to promote both cellular uptake and escape from the macropinosomes into the cytoplasm. Inhibition of macropinocytosis was sufficient to prevent conversion of PrPC to the pathologic PrPSc form, in N2a cells exposed to strain RML PrPSc infected brain homogenates, suggesting that a critical determinant of PrPC conversion occurs following macropinocytotic internalization and not through mere membrane association. Taken together, these observations provide a cellular, mechanism that exogenous pathological PrPSc infects cells by lipid raft dependent, macropinocytosis. © 2008 Wadia et Al.

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Wadia, J. S., Schaller, M., Williamson, R. A., & Dowdy, S. F. (2008). Pathologic prion protein infects cells by lipid-raft dependent macropinocytosis. PLoS ONE, 3(10). https://doi.org/10.1371/journal.pone.0003314

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