Immunosensors for the direct determination of proteins: Lactoferrin and HIgG

Abstract

New immunosensors for the direct determination of proteins, i.e. lactoferrin and human immunoglobulins G (HIgG), were developed. Direct measurement does not need any competition procedure; nevertheless, an enzymatic marker was used. For all the immunosensors developed, a systematic study was made to ensure their complete analytical characterisation using standard solutions of lactoferrin or HIgG, respectively. A comparison by the classical "competition" ELISA type immunosensors, studied in previous works, is also suggested. © 2010 Springer Science+Business Media B.V.