Saccharomyces cerevisiae is a useful eukaryotic expression system for mitochondrial membrane proteins due to its ease of growth and ability to provide a native membrane environment. The development of the pBEVY vector system has further increased the potential of S. cerevisiae as an expression system by creating a method for expressing multiple proteins simultaneously. This vector system is amenable to the expression and purification of multi-subunit protein complexes. Here we describe the cloning, yeast transformation, and co-expression of multi-subunit outer mitochondrial membrane complexes using the pBEVY vector system.
CITATION STYLE
Shaw, P. L. R., Diederichs, K. A., Pitt, A., Rollauer, S. E., & Buchanan, S. K. (2020). Cloning and Multi-Subunit Expression of Mitochondrial Membrane Protein Complexes in Saccharomyces cerevisiae. In Methods in Molecular Biology (Vol. 2127, pp. 1–11). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0373-4_1
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