Karakterisasi Bakteri Penambat N Asal Bayam Liar (Amaranthus spinosus L.) Sebagai Pemacu Perkecambahan Benih Bayam Hijau (Amaranthus spp. L.)

Abstract

Spinach required relatively high Nitrogen to grow it in less fertile fields. The use of free living N-fixing bacteria were expected to facilitate N supply to the crop. The research aims was to characterized of N-fixing bacterial isolates and its role to spinach germination. The exploration of bacterial isolates, were collected from wild spinach roots using LG and Ashby media. Bacterial isolates which were obtained, then being characterized based on its ability to dissolve P and produced indole compounds in Picovskaya and TSB media respectively, enriched with tryptophan. Superior isolates were used in the spinach germination test. Germination test using the UDK method in merang paper. The variables measured in this study were normal seedling, seedling vigor, root length, and height of normal seedling. The isolation results obtained 16 isolates that can fix N, 3 isolates (12BH3, 13BH4 and 1BH4) were higher in dissolving P and capable to produce indole compounds. Isolates 12BH3, 13BH3 and commercial isolates were used in the germination test. Test result showed that the bacteria can increase the N fixation, and high normal seedling sprouts.