A method is presented to measure homologous recombination in mouse embryonic stem cells by both gene targeting and short-tract gene conversion of a double-strand break (DSB). A fluorescence-based reporter is first gene targeted to the Hprt locus in a quantifiable way. A homing endonuclease expression vector is then introduced to generate a DSB, the repair of which is also quantifiable.
CITATION STYLE
Pierce, A. J., & Jasin, M. (2014). Measuring recombination proficiency in mouse embryonic stem cells. Methods in Molecular Biology, 1105, 481–495. https://doi.org/10.1007/978-1-62703-739-6_34
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