This chapter describes a method of plant cytosolic ribosomes isolation typically used for further proteomic studies. Detailed description procedures including plant material disruption, various centrifugation steps, sucrose cushion centrifugation, and quality control of preparation are provided.
CITATION STYLE
Årstrand, H. K., & Turkina, M. V. (2017). Isolation of Cytosolic Ribosomes. In Methods in Molecular Biology (Vol. 1511, pp. 241–247). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6533-5_19
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