Key message: This study elucidates the influence ofindehiscentmutations on rapeseed silique shatter resistance. A phenotype with enlarged replum-valve joint area and altered cell dimensions in the dehiscence zone is described. Abstract: Silique shattering is a major factor reducing the yield stability of oilseed rape (Brassica napus). Attempts to improve shatter resistance often include the use of mutations in target genes identified from Arabidopsis (Arabidopsis thaliana). A variety of phenotyping methods assessing the level of shatter resistance were previously described. However, a comparative and comprehensive evaluation of the methods has not yet been undertaken. We verified the increase of shatter resistance in indehiscent double knock-down mutants obtained by TILLING with a systematic approach comparing three independent phenotyping methods. A positive correlation of silique length and shatter resistance was observed and accounted for in the analyses. Microscopic studies ruled out the influence of different lignification patterns. Instead, we propose a model to explain increased shattering resistance of indehiscent rapeseed mutants by altered cell shapes and sizes within the contact surfaces of replum and valves.
CITATION STYLE
Braatz, J., Harloff, H. J., Emrani, N., Elisha, C., Heepe, L., Gorb, S. N., & Jung, C. (2018). The effect of INDEHISCENT point mutations on silique shatter resistance in oilseed rape (Brassica napus). Theoretical and Applied Genetics, 131(4), 959–971. https://doi.org/10.1007/s00122-018-3051-4
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