X-ray crystallography is the main technique for the determination of protein structures. About 85% of all protein structures known to date have been elucidated using X-ray crystallography. Knowledge of the three-dimensional structure of proteins can be used in various applications in biotechnology, biomedicine, drug design, and basic research and as a validation tool for protein modifications, ligand binding, and structural authenticity. Moreover, the requirement for pure, homogeneous, and stable protein solutions in crystallizations makes X-ray crystallography beneficial in other fields of protein research as well. Here, we describe the technique of X-ray protein crystallography and the steps involved for a successful three-dimensional crystal structure determination.
CITATION STYLE
Papageorgiou, A. C., & Mattsson, J. (2014). Protein structure validation and analysis with X-ray crystallography. Methods in Molecular Biology, 1129, 397–421. https://doi.org/10.1007/978-1-62703-977-2_29
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