Rapid In Vitro Proliferation of Tinospora Cordifolia Callus Biomass from Stem and Leaf with Phytochemical and Antimicrobial Assessment

Abstract

Tinospora cordifolia is a plant occurring from scrub to deciduous forest in various regions of India. The occurrence of this plant is limited in rainy season. It is broadly used in indigenous structure of medicine and there is an urgent need to discover new antimicrobial agents for therapeutic use. In current study, a procedure was developed for fast clonal dissemination of Tinospora cordifolia (TC) callus biomass via in vitro culture with the help of leaf, internodes, and nodal explants. Best results for leaf callus were observed on growth hormone 2,4, D (1 mg/l) alone. It was found that time taken by leaf for callusing is lower than any other part of the plant. Nodal segment gave best callusing on combination of BAP (1.5 mg/l) and 2,4, D (2 mg/l) which was further allowed to shooting on the MS media supplemented with BAP (2 mg/ml) and Kinetin (12 mg/l). Shoot tip when inoculated on media containing BAP (0.2 mg/lit) and Kinetin (0.1 mg/l) showed multiple shoots and growth of 6 mm in 25 days. Dried powder of callus was subjected to ethanolic, methanolic, and aqueous extraction. Extract was used for assessment of antimicrobial activity and phytochemical screening. Largest zone of inhibition was obtained against E. coli, followed by B. subtilis and P. aeruginosa.