Patients with pemphigus have circulating anti-desmoglein (Dsg) 1 and/or Dsg3 autoantibodies that induce blister formation on the skin and mucous membrane. We describe here two assays that measure the pathogenic strength of autoantibodies in blister formation: an in vitro dissociation assay using primary human epidermal keratinocytes to assess pathogenicity of anti-Dsg3 autoantibodies, and an alternative method whereby anti-Dsg3 and Dsg1 autoantibodies are injected into organ-cultured human skin specimen. © Springer Science+Business Media, LLC 2013.
CITATION STYLE
Ishii, K., & Amagai, M. (2013). In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus. Methods in Molecular Biology, 961, 219–225. https://doi.org/10.1007/978-1-62703-227-8_13
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