Bioluminescence from murine stem cells tagged with the luciferase gene reporter and distributed within three-dimensional scaffolds of two different materials is quantified in vitro and in vivo. The luminescence emitted from cells adhering to the scaffolds tested is monitored noninvasively using a bioluminescence imaging system. Monitoring the kinetics of luciferase expression via bioluminescence enables real-time assessment of cell survival and proliferation on scaffolds both in vitro and in vivo over prolonged (8 weeks) periods of time.
CITATION STYLE
Oudina, K., Cambon-Binder, A., & Logeart-Avramoglou, D. (2011). Noninvasive bioluminescent quantification of viable stem cells in engineered constructs. Methods in Molecular Biology (Clifton, N.J.), 740, 165–178. https://doi.org/10.1007/978-1-61779-108-6_18
Mendeley helps you to discover research relevant for your work.